Recombinant humaan Kallikrein 3/PSA-eiwit

Kallikrein 3, as known as prostate specific antigen (PSA), is a subgroup of serine proteases, enzymes capable of cleaving peptide bonds in proteins. This protein is expressed in the prostate and is thought to be responsible for regulating semen liquefaction through hydrolysis of seminogelin. It is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia. Recombinant human Kallikrein 3 protein, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme cleaves 1pmole of Succinyl-Arg-Pro-Tyr-p-Nitroanilide to Succinyl-Arg-Pro-Tyr and p-Nitroanilide per minute at pH7.5 at 37C.

KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, APS, hK3, KLK2A1, kallikrein related peptidase 3, prostate-specific antigen isoform 1

HEK293

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme cleaves 1pmole of Succinyl-Arg-Pro-Tyr-p-Nitroanilide to Succinyl-Arg-Pro-Tyr and p-Nitroanilide per minute at pH7.5 at 37C.

27.6 kDa (250aa)

Seminin, Semenogelase, PSA, Prostate-specific antigen isoform 1, Prostate-specific antigen, Prostate specific antigen isoform 1, Prostate specific antigen, P-30 antigen, P30 antigen, KLK3, KLK2A1, Kallikrein-3, Kallikrein3, Kallikrein related peptidase 3, Kallikrein 3, hK3, Gamma-seminoprotein, APS, Gamma seminoprotein, ATGP3926-10 µg, ATGP3926-20 µg, ATGP3926-50 µg, ATGP3926-100 µg, ATGP3926-250 µg, ATGP3926-500 µg, ATGP3926-1 mg, ATGP3926-010, ATGP3926-020, ATGP3926-050, ATGP3926-100, ATGP3926-250, ATGP3926-500, ATGP3926-01M

Hochmeister MN. et al. (1999) J Forensic Sci. J. 44:1057-1060. ; ; Jain, S. et al. (2002) Postgrad. Med. J. 78:646-650.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_001639.1

P07288

Human

Ala