Recombinant humaan Kallikreïne 10/KLK10-eiwit

KLK10, also known as kallikrein-10, is belong to the subgroup of serine proteases encompassing fifteen homologous members. This protein is widely expressed in normal human organs, for example breast, prostate, thyroid, testis, ovary, and gastrointestinal tract. Interestingly, it is highly expressed in the islets of Langerhans in human pancreas. Also, it is detectable in many biological fluids, including milk, seminal plasma, amniotic fluid, serum, and cerebrospinal fluid. In spite of its unknown physiological functions, the implications of KLK10 in various diseases have been explored. Early studies have shown that this expression is dramatically down-regulated in breast and testicular germ-cell tumors. It is secreted and may play a role in suppression of tumorigenesis in breast. Recombinant human KLK10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

PRSSL1, Protease serine-like 1, Normal epithelial cell-specific 1, NES1, KLK10, Kallikrein-10, Kallikrein related peptidase 10

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

27.8 kDa (252aa)

PRSSL1, Protease serine-like 1, Normal epithelial cell-specific 1, NES1, KLK10, Kallikrein-10, Kallikrein related peptidase 10, ATGP3834-10 µg, ATGP3834-20 µg, ATGP3834-50 µg, ATGP3834-100 µg, ATGP3834-250 µg, ATGP3834-500 µg, ATGP3834-1 mg, ATGP3834-010, ATGP3834-020, ATGP3834-050, ATGP3834-100, ATGP3834-250, ATGP3834-500, ATGP3834-01M

Petraki CD., et al, (2002) J. Histochem. Cytochem. 50:1247-1261.; ; Luo LY., et al. (2002) Br. J. Cancer. 86:1790-1796.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 1mM DTT, 30% glycerol

Signal Transduction

NP_001070968

O43240

Human

Ala

ADPALLPQND TRLDPEAYGS PCARGSQPWQ VSLFNGLSFH CAGVLVDQSW VLTAAHCGNK PLWARVGDDH LLLLQGEQLR RTTRSVVHPK YHQGSGPILP RRTDEHDLML LKLARPVVLG PRVRALQLPY RCAQPGDQCQ VAGWGTTAAR RVKYNKGLTC SSITILSPKE CEVFYPGVVT NNMICAGLDR GQDPCQSDSG GPLVCDETLQ GILSWGVYPC GSAQHPAVYT QICKYMSWIN KVIRSNHHHH HH