Recombinant humaan Kallikreïne 11/KLK11-eiwit

KLK11, also known as kallikrein-11 isoform 1, is belongs to the peptidases S1 family and Kallikrein subfamily. It is expressed in many tissues including cerebellum, prostate, salivary glands, stomach, lung, thymus, small intestine, spleen, liver, and uterus. These expression appear to be regulated by steroid hormones in the breast carcinoma cell line BT-474. Based on it serum levels, this protein has been proposed that ovarian cancer cases can be distinguished from healthy controls, who display very low expression. Also, it that is activated by thermolysin is active against thioester substrates such as dichloroisocoumarin and aprotinin. Recombinant human KLK11, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Kallikrein-11 isoform 2, Kallikrein related peptidase 11, KLK11, hK11, Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

26.7 kDa (241aa)

Kallikrein11, Kallikrein-11 isoform 1, KLK11, PRSS20, TLSP, ATGP3832-10 µg, ATGP3832-20 µg, ATGP3832-50 µg, ATGP3832-100 µg, ATGP3832-250 µg, ATGP3832-500 µg, ATGP3832-1 mg, ATGP3832-10, ATGP3832-20, ATGP3832-50, ATGP3832-100, ATGP3832-250, ATGP3832-500, ATGP3832-1

Mitsui S., et al, (2000) Biochem. Biophys. Res. Commun. 272:205-211.; ; Yoshida S., et al, (1998) Biochim. Biophys. Acta 1399:225-228.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 50 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 2mM CaCl2, 10% glycerol

Signal Transduction

NP_006844

Q9UBX7

Human

Ala

ADLETRIIKG FECKPHSQPW QAALFEKTRL LCGATLIAPR WLLTAAHCLK PRYIVHLGQH NLQKEEGCEQ TRTATESFPH PGFNNSLPNK DHRNDIMLVK MASPVSITWA VRPLTLSSRC VTAGTSCLIS GWGSTSSPQL RLPHTLRCAN ITIIEHQKCE NAYPGNITDT MVCASVQEGG KDSCQGDSGG PLVCNQSLQG IISWGQDPCA ITRKPGVYTK VCKYVDWIQE TMKNNHHHHH H