Recombinant humaan Kallikreïne 15/KLK15-eiwit

KLK15, also known as kallikrein-15 isoform 4, is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein contains multiple polyadenylation sites and alternative splicing results in multiple transcript variants encoding distinct isoforms. Also, growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. In prostate cancer, it has increased expression, which indicates its possible use as a diagnostic or prognostic marker for prostate cancer. Recombinant human KLK15, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Kallikrein-15, kallikrein related peptidase 15, ACO protease, HSRNASPH, prostinogen

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 80% by SDS-PAGE

27.4 kDa (249aa)

Prostinogen, KLK15, KLK 15, Kallikrein-related peptidase 15, Kallikrein-15 isoform 4 preproprotein, Kallikrein-15, Kallikrein15, Kallikrein 15, Kallikrein 15 isoform 4 preproprotein, Kallikrein related peptidase 15, HSRNASPH, ACO protease, ACO, ATGP3574-10 µg, ATGP3574-20 µg, ATGP3574-50 µg, ATGP3574-100 µg, ATGP3574-250 µg, ATGP3574-500 µg, ATGP3574-1 mg, ATGP3574-010, ATGP3574-020, ATGP3574-050, ATGP3574-100, ATGP3574-250, ATGP3574-500, ATGP3574-01M

Mavridis K., et al, (2013) Prostate 73:1191-1201.; ; Yousef GM., et al, (2001) J. Biol. Chem. 276:53-61.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_059979

Q9H2R5

Human

Ala

ADPQDGDKLL EGDECAPHSQ PWQVALYERG RFNCGASLIS PHWVLSAAHC QSRFMRVRLG EHNLRKRDGP EQLRTTSRVI PHPRYEARSH RNDIMLLRLV QPARLNPQVR PAVLPTRCPH PGEACVVSGW GLVSHNEPGT AGSPRSQVSL PDTLHCANIS IISDTSCDKS YPGRLTNTMV CAGAEGRGAE SCEGDSGGPL VCGGILQGIV SWGDVPCDNT TKPGVYTKVC HYLEWIRETM KRNHHHHHH