Recombinant humaan Kallikreïne 1/KLK1-eiwit

Kallikrein-1, also known as KLK1, belongs to the Kallikrein subfamily. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. Recombinant human KLK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.7 kDa (259aa) confirmed by MALDI-TOF

KLK1, Kallikrein-1, hK1, Klk6, KLKR, ATGP1766-10 µg, ATGP1766-20 µg, ATGP1766-50 µg, ATGP1766-100 µg, ATGP1766-250 µg, ATGP1766-500 µg, ATGP1766-1 mg, ATGP1766-10, ATGP1766-20, ATGP1766-50, ATGP1766-100, ATGP1766-250, ATGP1766-500, ATGP1766-1

Lu H.S., et al. (1989) Int. J. Pept. Protein Res. 33:237-249; ; Kellermann J., et al. (1988) Protein Seq. Data Anal. 1:177-182

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cancer

NP_002248

P06870

Human

MGSSHHHHHH SSGLVPRGSH MIVGGWECEQ HSQPWQAALY HFSTFQCGGI LVHRQWVLTA AHCISDNYQL WLGRHNLFDD ENTAQFVHVS ESFPHPGFNM SLLENHTRQA DEDYSHDLML LRLTEPADTI TDAVKVVELP TQEPEVGSTC LASGWGSIEP ENFSFPDDLQ CVDLKILPND ECKKVHVQKV TDFMLCVGHL EGGKDTCVGD SGGPLMCDGV LQGVTSWGYV PCGTPNKPSV AVRVLSYVKW IEDTIAENS