Recombinant humaan Kallikreïne 2/KLK2-eiwit

KLK2 is a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. KLK2 is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. Recombinant human KLK2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Kallikrein related peptidase 2, Glandular kallikrein-1, hGK-1, Tissue kallikrein-2, Prostatic kallikrein 2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

28.5 kDa (260aa)

Kallikrein2, Kallikrein-related peptidase 2, hK2, KLK2A2, ATGP2175-10 µg, ATGP2175-20 µg, ATGP2175-50 µg, ATGP2175-100 µg, ATGP2175-250 µg, ATGP2175-500 µg, ATGP2175-1 mg, ATGP2175-10, ATGP2175-20, ATGP2175-50, ATGP2175-100, ATGP2175-250, ATGP2175-500, ATGP2175-1

Cargill M., et al. (1999) Nat. Genet. 22:231-238; ; Gan L., et al. (2000) Gene. 257:119-130

Lee SW, et al. Simple and robust antibody microarray-based immunoassay platform for sensitive and selective detection of PSA and hK2 toward accurate diagnosis of prostate cancer. (Sensing and Bio-Sensing Research, 2015) {https://doi.org/10.1016/j.sbsr.2015.01.003.}; ; Lee SW, et al. A Highly Sensitive Porous Silicon (P-Si) -Based Human Kallikrein 2 (hK2) Immunoassay Platform toward Accurate Diagnosis of Prostate Cancer. (Sensors (Basel) . 2015) {https://www.ncbi.nlm.nih.gov/pubmed/26007739}; ; Lee SW, et al. Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2. (Microchim Acta. 2016) {https://doi.org/10.1007/s00604-016-1986-1}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Cancer

NP_005542

P20151

Human

MGSSHHHHHH SSGLVPRGSH MGSIVGGWEC EKHSQPWQVA VYSHGWAHCG GVLVHPQWVL TAAHCLKKNS QVWLGRHNLF EPEDTGQRVP VSHSFPHPLY NMSLLKHQSL RPDEDSSHDL MLLRLSEPAK ITDVVKVLGL PTQEPALGTT CYASGWGSIE PEEFLRPRSL QCVSLHLLSN DMCARAYSEK VTEFMLCAGL WTGGKDTCGG DSGGPLVCNG VLQGITSWGP EPCALPEKPA VYTKVVHYRK WIKDTIAANP