Recombinant humaan Kallikreïne 5/KLK5-eiwit

KLK5, also known as kallikrein-5, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. It is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14. Recombinant human KLK5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Kallikrein-5, KLK5, KLKL2, SCTE, Kallikrein related peptidase 5, Kallikrein-like protein 2, Stratum corneum tryptic enzyme

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

26.2 kDa (236aa)

Kallikrein-5, KLK5, KLKL2, KLK-L2, SCTE, Kallikrein related peptidase 5, Kallikrein-like protein 2, Stratum corneum tryptic enzyme, ATGP3501-10 µg, ATGP3501-20 µg, ATGP3501-50 µg, ATGP3501-100 µg, ATGP3501-250 µg, ATGP3501-500 µg, ATGP3501-1 mg, ATGP3501-010, ATGP3501-020, ATGP3501-050, ATGP3501-100, ATGP3501-250, ATGP3501-500, ATGP3501-01M

Kim H., et al. (2001) Br J Cancer. 84:643-650.; ; Pampalakis G., et al. (2014) Oncotarget. 5:2390-2403.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001070960.1

Q9Y337

Human

Ala

IINGSDC DMHTQPWQAA LLLRPNQLYC GAVLVHPQWL LTAAHCRKKV FRVRLGHYSL SPVYESGQQM FQGVKSIPHP GYSHPGHSND LMLIKLNRRI RPTKDVRPIN VSSHCPSAGT KCLVSGWGTT KSPQVHFPKV LQCLNISVLS QKRCEDAYPR QIDDTMFCAG DKAGRDSCQG DSGGPVVCNG SLQGLVSWGD YPCARPNRPG VYTNLCKFTK WIQETIQANS