Recombinant humaan KAP1-eiwit

TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Recombinant human TRIM28 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Transcription intermediary factor 1-beta, Transcription intermediary factor 1-beta, Tripartite motif containing 28, KAP1, RNF96, TF1B, TIF1B

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

48.7 kDa (460aa)

KAP1, Transcription intermediary factor 1-beta, Transcription intermediary factor 1-beta, Tripartite motif containing 28, KAP1, RNF96, TF1B, TIF1B, ATGP2325-10 µg, ATGP2325-20 µg, ATGP2325-50 µg, ATGP2325-100 µg, ATGP2325-250 µg, ATGP2325-500 µg, ATGP2325-1 mg, ATGP2325-10, ATGP2325-20, ATGP2325-50, ATGP2325-100, ATGP2325-250, ATGP2325-500, ATGP2325-1

Friedman J.R, . et al. (1996) . Genes Dev. 10:2067-2078

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Epigenomics (Transcription & Translation)

NP_005753

Q13263

Human

KLIYFQL HRALKMIVDP VEPHGEMKFQ WDLNAWTKSA EAFGKIVAER PGTNSTGPAP MAPPRAPGPL SKQGSGSSQP MEVQEGYGFG SGDDPYSSAE PHVSGVKRSR SGEGEVSGLM RKVPRVSLER LDLDLTADSQ PPVFKVFPGS TTEDYNLIVI ERGAAAAATG QPGTAPAGTP GAPPLAGMAI VKEEETEAAI GAPPTATEGP ETKPVLMALA EGPGAEGPRL ASPSGSTSSG LEVVAPEGTS APGGGPGTLD DSATICRVCQ KPGDLVMCNQ CEFCFHLDCH LPALQDVPGE EWSCSLCHVL PDLKEEDGSL SLDGADSTGV VAKLSPANQR KCERVLLALF CHEPCRPLHQ LATDSTFSLD QPGGTLDLTL IRARLQEKLS PPYSSPQEFA QDVGRMFKQF NKLTEDKADV QSIIGLQRFF ETRMNEAFGD