Recombinant humaan KARS-eiwit

KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Lysyl-tRNA synthetase, CMTRIB, KARS2, KIAA0070, KRS

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

63.7 kDa (558aa)

KARS, Lysyl-tRNA synthetase, CMTRIB, KARS2, KIAA0070, KRS, ATGP1166-10 µg, ATGP1166-20 µg, ATGP1166-50 µg, ATGP1166-100 µg, ATGP1166-250 µg, ATGP1166-500 µg, ATGP1166-1 mg, ATGP1166-10, ATGP1166-20, ATGP1166-50, ATGP1166-100, ATGP1166-250, ATGP1166-500, ATGP1166-1

Targoff I.N. et al. (1993) J. Clin. Invest. 91: 2556-2564.; ; Tolkunova E. et al. (2000) J. Biol. Chem. 275: 35063-35069.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Enzymes & Proteases

NP_005539.1

Q15046

Human

GVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV