Recombinant humaan KAT2A/GCN5-eiwit

GCN5L2 (General control of amino acid synthesis protein 5 like 2) belongs to the GCN5 family. It functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. GCN5L2 has been shown to interact with Ku70, TAF9, transcription initiation protein SPT3 homolog, TADA2L, Ku80 and DDB1. Recombinant human GCN5L2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

STAF97, PCAF-b, Lysine acetyltransferase 2A, Histone succinyltransferase KAT2A, Histone glutaryltransferase KAT2A, Histone acetyltransferase KAT2A, Histone acetyltransferase GCN5, General control of amino-acid synthesis 5-like 2, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

51.1 kDa (447a) confirmed by MALDI-TOF

STAF97, PCAF-b, Lysine acetyltransferase 2A, KAT2A, K (lysine) acetyltransferase 2A, Histone succinyltransferase KAT2A, Histone glutaryltransferase KAT2A, Histone acetyltransferase KAT2A, Histone acetyltransferase GCN5, hGCN5L2, hGCN5, General control of amino-acid synthesis 5-like 2, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, ATGP1182-10 µg, ATGP1182-20 µg, ATGP1182-50 µg, ATGP1182-100 µg, ATGP1182-250 µg, ATGP1182-500 µg, ATGP1182-1 mg, ATGP1182-010, ATGP1182-020, ATGP1182-050, ATGP1182-100, ATGP1182-250, ATGP1182-500, ATGP1182-01M

Guelman S., et al. (2009) Mol. Cell. Biol. 29:1176-1188; ; Col E., et al. (2001) J. Biol. Chem. 276:28179-28184

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 40% glycerol, 200 mM NaCl, 1mM EDTA

Epigenomics (Transcription & Translation)

NP_066564.2

Q92830

Human

MGGGSNSSLS LDSAGAEPMP GEKRTLPENL TLEDAKRLRV MGDIPMELVN EVMLTITDPA AMLGPETSLL SANAARDETA RLEERRGIIE FHVIGNSLTP KANRRVLLWL VGLQNVFSHQ LPRMPKEYIA RLVFDPKHKT LALIKDGRVI GGICFRMFPT QGFTEIVFCA VTSNEQVKGY GTHLMNHLKE YHIKHNILYF LTYADEYAIG YFKKQGFSKD IKVPKSRYLG YIKDYEGATL MECELNPRIP YTELSHIIKK QKEIIKKLIE RKQAQIRKVY PGLSCFKEGV RQIPVESVPG IRETGWKPLG KEKGKELKDP DQLYTTLKNL LAQIKSHPSA WPFMEPVKKS EAPDYYEVIR FPIDLKTMTE RLRSRYYVTR KLFVADLQRV IANCREYNPP DSEYCRCASA LEKFFYFKLK EGGLIDK