Recombinant humaan KDSR-eiwit

KDSR, also known as 3-ketodihydrosphingosine reductase, is a 332 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum (ER) and belongs to the short-chain dehydrogenases/reductases (SDR) family. KDSR is a secreted protein which is weakly expressed in hematopoietic tissue. Also this protein catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS) Recombinant human KDSR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

3-ketodihydrosphingosine reductase, DHSR, FVT1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.0 kDa (266aa) confirmed by MALDI-TOF

KDSR, 3-ketodihydrosphingosine reductase, DHSR, FVT1, ATGP0948-10 µg, ATGP0948-20 µg, ATGP0948-50 µg, ATGP0948-100 µg, ATGP0948-250 µg, ATGP0948-500 µg, ATGP0948-1 mg, ATGP0948-10, ATGP0948-20, ATGP0948-50, ATGP0948-100, ATGP0948-250, ATGP0948-500, ATGP0948-1

Kihara A., et al. (2004) J. Biol. Chem. 279:49243-49250; ; Parkinson, N.J., et al. (2008) Neuromuscul. Disord. 18: 394-397.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl, 0.1mM PMSF

Metabolism

NP_002026

Q06136

Human

KPLALPGAH VVVTGGSSGI GKCIAIECYK QGAFITLVAR NEDKLLQAKK EIEMHSINDK QVVLCISVDV SQDYNQVENV IKQAQEKLGP VDMLVNCAGM AVSGKFEDLE VSTFERLMSI NYLGSVYPSR AVITTMKERR VGRIVFVSSQ AGQLGLFGFT AYSASKFAIR GLAEALQMEV KPYNVYITVA YPPDTDTPGF AEENRTKPLE TRLISETTSV CKPEQVAKQI VKDAIQGNFN SSLGSD