Recombinant humaan Keratine 14/KRT14-eiwit

KRT14, also known as keratin 14, is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs) . It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis. Recombinant human KRT14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

53.8 kDa (492aa)

KRT14, Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI, ATGP1225-10 µg, ATGP1225-20 µg, ATGP1225-50 µg, ATGP1225-100 µg, ATGP1225-250 µg, ATGP1225-500 µg, ATGP1225-1 mg, ATGP1225-10, ATGP1225-20, ATGP1225-50, ATGP1225-100, ATGP1225-250, ATGP1225-500, ATGP1225-1

Romano RA. et al. (2007) J Invest Dermatol. 127:1175-1186.; ; Coulombe PA. et al. (1991) Cell. 66 (6) : 1301-11.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Cytoskeleton

AAH19097

P02533

Human

MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN