Recombinant humaan Keratine 8/KRT8-eiwit

KRT8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Recombinant human KRT8 protein, fused to His-tag at N-terminus, was expressed in E. coli.

KO, Keratin type II cytoskeletal 8, Keratin 8, K8, K2C8, CYK8, CK8, CARD2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

56.1 kDa (506aa)

Type II cytoskeletal 8, KO, Keratin8, Keratin type II cytoskeletal 8, Keratin 8, Keratin, K-8, K8, K2C-8, K2C8, K2C 8, K 8, Cytokeratin8, Cytokeratin 8, CYK8, CYK 8, CK-8, CK8, CK 8, CARD-2, CARD2, CARD 2, ATGP2076-10 µg, ATGP2076-20 µg, ATGP2076-50 µg, ATGP2076-100 µg, ATGP2076-250 µg, ATGP2076-500 µg, ATGP2076-1 mg, ATGP2076-010, ATGP2076-020, ATGP2076-050, ATGP2076-100, ATGP2076-250, ATGP2076-500, ATGP2076-01M

Babichenko II, Grigor'ian AS et al. Arkh Patol. 2011 Nov-Dec; ;73 (6) :18-21.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Cytoskeleton

NP_002264.1

P05787

Human

MSIRVTQ KSYKVSTSGP RAFSSRSYTS GPGSRISSSS FSRVGSSNFR GGLGGGYGGA SGMGGITAVT VNQSLLSPLV LEVDPNIQAV RTQEKEQIKT LNNKFASFID KVRFLEQQNK MLETKWSLLQ QQKTARSNMD NMFESYINNL RRQLETLGQE KLKLEAELGN MQGLVEDFKN KYEDEINKRT EMENEFVLIK KDVDEAYMNK VELESRLEGL TDEINFLRQL YEEEIRELQS QISDTSVVLS MDNSRSLDMD SIIAEVKAQY EDIANRSRAE AESMYQIKYE ELQSLAGKHG DDLRRTKTEI SEMNRNISRL QAEIEGLKGQ RASLEAAIAD AEQRGELAIK DANAKLSELE AALQRAKQDM ARQLREYQEL MNVKLALDIE IATYRKLLEG EESRLESGMQ NMSIHTKTTS GYAGGLSSAY GGLTSPGLSY SLGSSFGSGA GSSSFSRTSS SRAVVVKKIE TRDGKLVSES SDVLPK