Recombinant humaan Ketohexokinase-eiwit

Ketohexokinase is an enzyme that catalyzes the phosphorylation of fructose to produce fructose-1-phosphate, leading to consumption of ATP, formation of AMP. This protein initiates first step in the metabolism of dietary fructose and is an important regulator of hepatic glucose metabolism. It is highly found in liver, renal cortex, and small intestine. Its deficiency causes the benign hereditary metabolic disorder essential fructosuria, leading to fructose being excreted in the urine. Recombinant human Ketohexokinase was expressed in E. coli and purified by using conventional chromatography.

KHK, Hepatic fructokinase, EC 2.7.1.3, Hepatic fructokinase, ketohexokinase, Ketohexokinase isoform a

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

32.7 kDa (298aa) confirmed by MALDI-TOF

Ketohexokinase, KHK, Hepatic fructokinase, EC 2.7.1.3, Hepatic fructokinase, ketohexokinase, Ketohexokinase isoform a, ATGP0437-10 µg, ATGP0437-20 µg, ATGP0437-50 µg, ATGP0437-100 µg, ATGP0437-250 µg, ATGP0437-500 µg, ATGP0437-1 mg, ATGP0437-10, ATGP0437-20, ATGP0437-50, ATGP0437-100, ATGP0437-250, ATGP0437-500, ATGP0437-1

Bonthron DT., et al. (2009) J Histochem Cytochem. 57 (8) :763-74.; ; Schermerhorn T., et al. (2009) Res Vet Sci. 87 (1) :115-7. ;

Wuest M, et al. Radiopharmacological evaluation of 6-deoxy-6-[18F]fluoro-D-fructose as a radiotracer for PET imaging of GLUT5 in breast cancer. (Nucl Med Biol. 2011) {https://pubmed.ncbi.nlm.nih.gov/21531283/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

AAH06233

P50053

Human

MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTIL SLLGAPCAFM GSMAPGHVAD FVLDDLRRYS VDLRYTVFQT TGSVPIATVI INEASGSRTI LYYDRSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIV