Recombinant humaan KIAA0101/PCLAF-eiwit

KIAA0101 is a PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. This protein also acts as a regulator of centrosome number. Recombinant human KIAA0101 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

PCNA clamp associated factor, PAF15, PAF, p15 (PAF), OEATC-1, OEATC, NS5ATP9, L5, KIAA0101

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.4 kDa (134aa) confirmed by MALDI-TOF

PCNA clamp associated factor, PAF-15, PAF15, PAF 15, PAF, p15-PAF, p15PAF, p15/PAF, p15 (PAF), p15 PAF, OEATC-1, OEATC1, OEATC, NS5ATP9, L5, KIAA0101, ATGP2304-10 µg, ATGP2304-20 µg, ATGP2304-50 µg, ATGP2304-100 µg, ATGP2304-250 µg, ATGP2304-500 µg, ATGP2304-1 mg, ATGP2304-010, ATGP2304-020, ATGP2304-050, ATGP2304-100, ATGP2304-250, ATGP2304-500, ATGP2304-01M

Kais Z., et al (2011) . Mol. Cancer Res. 9:1091-1099; ; Povlsen L.K., et al (2012) . Nat. Cell Biol. 14:1089-1098

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_055551.1

Q15004

Human

MVRTKAD SVPGTYRKVV AARAPRKVLG SSTSATNSTS VSSRKAENKY AGGNPVCVRP TPKWQKGIGE FFRLSPKDSE KENQIPEEAG SSGLGKAKRK ACPLQPDHTN DEKE