Recombinant humaan KIBRA/WWC1-eiwit

WWC1 is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of WWC1 have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human WWC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

HBeAg-binding protein 3, Kidney and brain protein, KIBRA, WW domain-containing protein 1, WWC1, KIAA0869, PPP1R168, Protein phosphatase 1, Regulatory subunit 168

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Absorbance at 280 nm)

> 90% by SDS-PAGE

17 kDa (152aa) confirmed by MALDI-TOF

HBeAg binding protein 3, HBeAg-binding protein 3, HBEBP 3, HBEBP 36, HBEBP3, HBEBP36, KIAA 0869, KIAA0869, KIBRA, Kidney and brain protein, PPP1R168, Protein KIBRA isoform 3, Protein phosphatase 1, Protein-KIBRA-isoform 3, Protein-phosphatase 1, Regulatory subunit 168, Regulatory subunit168, WW and C2 domain containing 1, WW and C2 domain-containing 1, WW domain containing protein 1, WW domain-containing protein 1, WWC 1, WWC1, ATGP2549-10 µg, ATGP2549-20 µg, ATGP2549-50 µg, ATGP2549-100 µg, ATGP2549-250 µg, ATGP2549-500 µg, ATGP2549-1 mg, ATGP2549-010, ATGP2549-020, ATGP2549-050, ATGP2549-100, ATGP2549-250, ATGP2549-500, ATGP2549-01M

Buether K., Plaas C., et al. (2004) Biochem. Biophys. Res. Commun. 317:703-707

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 1mM DTT, 20% glycerol

Signal Transduction

NP_056053.1

Q8IX03

Human

EAVGATR IQIALKYDEK NKQFAILIIQ LSNLSALLQQ QDQKVNIRVA VLPCSESTTC LFRTRPLDAS DTLVFNEVFW VSMSYPALHQ KTLRVDVCTT DRSHLEECLG GAQISLAEVC RSGERSTRWY NL