Recombinant humaan KLF12-eiwit

KLF12, as known as Krueppel-like factor 12, is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene. cers. Recombinant human KLF12 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Krueppel-like factor 12, AP-2rep, AP2REP, HSPC122

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

46.6 kDa (425aa)

KLF12, Krueppel-like factor 12, AP-2rep, AP2REP, HSPC122, ATGP2825-10 µg, ATGP2825-20 µg, ATGP2825-50 µg, ATGP2825-100 µg, ATGP2825-250 µg, ATGP2825-500 µg, ATGP2825-1 mg, ATGP2825-10, ATGP2825-20, ATGP2825-50, ATGP2825-100, ATGP2825-250, ATGP2825-500, ATGP2825-1

Roth C. et al. (1996) Genomics. 63:384-390.; ; Schuierer M. et al. (2001) J Biol Chem. 276:27944-27949.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea

Epigenomics (Transcription & Translation)

NP_009180

Q9Y4X4

Human

MNIHMKR KTIKNINTFE NRMLMLDGMP AVRVKTELLE SEQGSPNVHN YPDMEAVPLL LNNVKGEPPE DSLSVDHFQT QTEPVDLSIN KARTSPTAVS SSPVSMTASA SSPSSTSTSS SSSSRLASSP TVITSVSSAS SSSTVLTPGP LVASASGVGG QQFLHIIHPV PPSSPMNLQS NKLSHVHRIP VVVQSVPVVY TAVRSPGNVN NTIVVPLLED GRGHGKAQMD PRGLSPRQSK SDSDDDDLPN VTLDSVNETG STALSIARAV QEVHPSPVSR VRGNRMNNQK FPCSISPFSI ESTRRQRRSE SPDSRKRRIH RCDFEGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK FARSDELTRH YRKHTGVKPF KCADCDRSFS RSDHLALHRR RHMLV