Recombinant humaan KLF6-eiwit

KLF6, also known as Krueppel-like factor 6, is a nuclear protein that has three zinc fingers. The zinc fingers of this protein are responsible for the specific DNA binding with the guanine-rich core promoter elements. The central region might be involved in activation or posttranslational regulatory pathways, and the acidic N-terminal domain might play an important role in the process of transcriptional activation. It is capable of activating transcription approximately 4-fold either on homologous or heterologous promoters. Recombinant human KLF6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Kruppel-like factor 6, Kruppel-like factor 6, B-cell-derived protein 1, BCD1, CBA1, COPEB, CPBP, Kruppel like factor 6, core promoter element binding protein, GBF, GC-rich binding factor, PAC1, Zf9

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.3 kDa (306aa) confirmed by MALDI-TOF

KLF6, ATGP2550-10 µg, ATGP2550-20 µg, ATGP2550-50 µg, ATGP2550-100 µg, ATGP2550-250 µg, ATGP2550-500 µg, ATGP2550-1 mg, ATGP2550-10, ATGP2550-20, ATGP2550-50, ATGP2550-100, ATGP2550-250, ATGP2550-500, ATGP2550-1

Narla G. et al. (2003) Am J Pathol. 162:1047-1052.; ; Narla G. et al. (2001) Science. 294:2563-2566;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Stem cell

NP_001291

Q99612

Human

MDVLPMC SIFQELQIVH ETGYFSALPS LEEYWQQTCL ELERYLQSEP CYVSASEIKF DSQEDLWTKI ILAREKKEES ELKISSSPPE DTLISPSFCY NLETNSLNSD VSSESSDSSE ELSPTAKFTS DPIGEVLVSS GKLSSSVTST PPSSPELSRE PSQLWGCVPG ELPSPGKVRS GTSGKPGDKG NGDASPDGRR RVHRCHFNGC RKVYTKSSHL KAHQRTHTGE KPYRCSWEGC EWRFARSDEL TRHFRKHTGA KPFKCSHCDR CFSRSDHLAL HMKRHL