Recombinant humaan KMT5A-eiwit

KMT5A, also known as N-lysine methyltransferase KMT5A isoform 1, is an enzyme which catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1) . It is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. It acts as a barrier to prevent cellular senescence through chromatin-mediated regulation of senescence-associated metabolic remodeling. It mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. The loss of KMT5A concurrently stimulate nucleolar function and retinoblastoma protein-mediated mitochondrial metabolism. Recombinant human KMT5A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

N-lysine methyltransferase KMT5A isoform 1, KMT5A, PR-Set7, SET07, SET8, SETD8

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

18.9 kDa (165aa)

KMT5A, N-lysine methyltransferase KMT5A isoform 1, KMT5A, PR-Set7, SET07, SET8, SETD8, ATGP3812-10 µg, ATGP3812-20 µg, ATGP3812-50 µg, ATGP3812-100 µg, ATGP3812-250 µg, ATGP3812-500 µg, ATGP3812-1 mg, ATGP3812-10, ATGP3812-20, ATGP3812-50, ATGP3812-100, ATGP3812-250, ATGP3812-500, ATGP3812-1

Nishioka K., et al. (2002) Mol Cell. 9:1201-1213.; ; Tanaka H., et al. (2017) Cell Rep. 18:2148-2161.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 0.2M NaCl, 1mM EDTA, 50% glycerol

Epigenomics (Transcription & Translation)

NP_065115

Q9NQR1

Human

Met

MKAELQSEER KRIDELIESG KEEGMKIDLI DGKGRGVIAT KQFSRGDFVV EYHGDLIEIT DAKKREALYA QDPSTGCYMY YFQYLSKTYC VDATRETNRL GRLINHSKCG NCQTKLHDID GVPHLILIAS RDIAAGEELL YDYGDRSKAS IEAHPWLKHH HHHHH