Recombinant humaan koolzuuranhydrase 11/CA11-eiwit

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA11 is likely a secreted protein, however, radical changes at active site residues completely conserved in CA isozymes with catalytic activity, make it unlikely that it has carbonic anhydrase activity. It shares properties in common with two other acatalytic CA isoforms, CA VIII and CA X. CA11 is most abundantly expressed in brain, and may play a general role in the central nervous system. Recombinant human CA11 protein, fused to His-tag at N-terminus, was expressed in E. coli.

CA-XI, CARPX1, CARP2, CA-RP XI, carbonic anhydrase-related protein XI, carbonic anhydrase-related protein 2, Carbonic anhydrase-related protein 11, Carbonic anhydrase XI

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.3 kDa (326aa)

CA11, Carbonic anhydrase-related protein 11, CARPX1, ATGP2219-10 µg, ATGP2219-20 µg, ATGP2219-50 µg, ATGP2219-100 µg, ATGP2219-250 µg, ATGP2219-500 µg, ATGP2219-1 mg, ATGP2219-10, ATGP2219-20, ATGP2219-50, ATGP2219-100, ATGP2219-250, ATGP2219-500, ATGP2219-1

Fujikawa-Adachi K., et al. (1999) Biochim. Biophys. Acta. 1431:518-524

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Enzymes & Proteases

NP_001208

O75493

Human

MGSSHHHHHH SSGLVPRGSH MHIGPAPDPE DWWSYKDNLQ GNFVPGPPFW GLVNAAWSLC AVGKRQSPVD VELKRVLYDP FLPPLRLSTG GEKLRGTLYN TGRHVSFLPA PRPVVNVSGG PLLYSHRLSE LRLLFGARDG AGSEHQINHQ GFSAEVQLIH FNQELYGNFS AASRGPNGLA ILSLFVNVAS TSNPFLSRLL NRDTITRISY KNDAYFLQDL SLELLFPESF GFITYQGSLS TPPCSETVTW ILIDRALNIT SLQMHSLRLL SQNPPSQIFQ SLSGNSRPLQ PLAHRALRGN RDPRHPERRC RGPNYRLHVD GVPHGR