Recombinant humaan koolzuuranhydrase 12/CA12 eiwit

CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Carbonic anhydrase 12 isoform 1, CAXII, HsT18816, Carbonate dehydratase XII, CA-XII, tumor antigen HOM-RCC-3.1.3, Carbonic Anhydrase XII

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

31.94 kDa (283aa)

Carbonic Anhydrase XII, Carbonic anhydrase 12 isoform 1, CAXII, HsT18816, ATGP3124-10 µg, ATGP3124-20 µg, ATGP3124-50 µg, ATGP3124-100 µg, ATGP3124-250 µg, ATGP3124-500 µg, ATGP3124-1 mg, ATGP3124-10, ATGP3124-20, ATGP3124-50, ATGP3124-100, ATGP3124-250, ATGP3124-500, ATGP3124-1

Tureci O. et al. (1998) Proc Natl Acad Sci U S A. 95 (13) :7608-13.; ; Kyllonen MS. et al. (2003) J Histochem Cytochem. 51 (9) :1217-24.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cancer

NP_001209.1

O43570

Human