Recombinant humaan koolzuuranhydrase 14/CA14 eiwit

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. Recombinant human CA14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Carbonate dehydratase XIV, Carbonic anhydrase XIV, CA-XIV

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

33.2 kDa (298aa) confirmed by MALDI-TOF

Carbonate dehydratase 14, Carbonate dehydratase XIV, Carbonic anhydrase 14, Carbonic anhydrase XIV, CAXiV, CA-XIV, CA14, ATGP2485-10 µg, ATGP2485-20 µg, ATGP2485-50 µg, ATGP2485-100 µg, ATGP2485-250 µg, ATGP2485-500 µg, ATGP2485-1 mg, ATGP2485-010, ATGP2485-020, ATGP2485-050, ATGP2485-100, ATGP2485-250, ATGP2485-500, ATGP2485-01M

Temperini C., et al. (2006) Chemistry. 12:7057-7066; ; Temperini C., et al. (2006) J. Med. Chem. 49:3019-3027;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Neuroscience

NP_036245

Q9ULX7

Human

MGSSHHHHHH SSGLVPRGSH MGSADGGQHW TYEGPHGQDH WPASYPECGN NAQSPIDIQT DSVTFDPDLP ALQPHGYDQP GTEPLDLHNN GHTVQLSLPS TLYLGGLPRK YVAAQLHLHW GQKGSPGGSE HQINSEATFA ELHIVHYDSD SYDSLSEAAE RPQGLAVLGI LIEVGETKNI AYEHILSHLH EVRHKDQKTS VPPFNLRELL PKQLGQYFRY NGSLTTPPCY QSVLWTVFYR RSQISMEQLE KLQGTLFSTE EEPSKLLVQN YRALQPLNQR MVFASFIQAG SSYTTGEM