Recombinant humaan koolzuuranhydrase 1/CA1-eiwit

Carbonic anhydrase 1 (CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O <-> HCO3- + H+) . This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Carbonic anhydrase I, CA-I, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Cyanamide hydratase CA1, Car1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.0 kDa (281aa) confirmed by MALDI-TOF

Carbonic Anhydrase I, CA1, CA1, CA-I, CAB, JW0122, CA 1, CA I, CAI, CAN, Car 1, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, Carbonic dehydratase, ECK0125, yadF, ATGP0427-10 µg, ATGP0427-20 µg, ATGP0427-50 µg, ATGP0427-100 µg, ATGP0427-250 µg, ATGP0427-500 µg, ATGP0427-1 mg, ATGP0427-10, ATGP0427-20, ATGP0427-50, ATGP0427-100, ATGP0427-250, ATGP0427-500, ATGP0427-1

Nogradi A., et al. (1998) . Am J Pathol. 153:1-4; ; Ferry JG., et al. (1999) . Proc Natl Acad Sci u S A. 96 (26) :15184-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_001122301

P00915

Human

MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F