Recombinant humaan koolzuuranhydrase 1/CA1-eiwit

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3- + H+) . Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques.

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Carbonic anhydrase I, CA-I, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Cyanamide hydratase CA1, Car1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

31.0 kDa (281aa) confirmed by MALDI-TOF

Carbonic Anhydrase I, CA1, CA-I, CAB, ATGP3241-10 µg, ATGP3241-20 µg, ATGP3241-50 µg, ATGP3241-100 µg, ATGP3241-250 µg, ATGP3241-500 µg, ATGP3241-1 mg, ATGP3241-10, ATGP3241-20, ATGP3241-50, ATGP3241-100, ATGP3241-250, ATGP3241-500, ATGP3241-1

Nogradi A., et al. (1998) . Am J Pathol. 153:1-4.; ; Ferry JG., et al. (1999) . Proc Natl Acad Sci U S A. 96 (26) :15184-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_001122301

P00915

Human

MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F