Recombinant humaan koolzuuranhydrase 2/CA2-eiwit

Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Carbonic anhydrase II, CA-II, Carbonate dehydratase II, Carbonic anhydrase C, CAC, Cyanamide hydratase CA2, Car2

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 7,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

29.2 kDa (260aa) confirmed by MALDI-TOF

Carbonic Anhydrase II, CA2, Carbonic anhydrase 2, CA-II, CAC, CA 2, CA II, CA2, CAI, Car 2, Car2, Carbonate dehydratase II, Carbonic anhydrase B, Carbonic anhydrase C, Carbonic anhydrase II, Carbonic dehydratase., ATGP0325-10 µg, ATGP0325-20 µg, ATGP0325-50 µg, ATGP0325-100 µg, ATGP0325-250 µg, ATGP0325-500 µg, ATGP0325-1 mg, ATGP0325-10, ATGP0325-20, ATGP0325-50, ATGP0325-100, ATGP0325-250, ATGP0325-500, ATGP0325-1

Hu PY., et al. (1992) . Hum Mutat. 1 (4) :288-92 ; ; Venta PJ., et al. (1991) . Am J Hum Genet. 49 (5) :1082-90;

Saito M, et al. A case of paraneoplastic optic neuropathy and outer retinitis positive for autoantibodies against collapsin response mediator protein-5, recoverin, and α-enolase. (BMC Ophthalmol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24428923/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_000058.1

P00918

Human

MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK