Recombinant humaan koolzuuranhydrase 3/CA3 eiwit

CA3, also known as carbonic anhydrase III, is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O = HCO3 + H+) . This protein is involved in a variety of biological processes, including respiration, calcifica-tion, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. It contains a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Recombinant CA3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Carbonic anhydrase III, Carbonate dehydratase III, Car3, CAIII, CA3

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.5 kDa (260aa) confirmed by MALDI-TOF

Carbonic Anhydrase III, CA3, Carbonic anhydrase III, CAIII, Car3, Carbonic anhydrase III, CA3, ATGP0503-10 µg, ATGP0503-20 µg, ATGP0503-50 µg, ATGP0503-100 µg, ATGP0503-250 µg, ATGP0503-500 µg, ATGP0503-1 mg, ATGP0503-10, ATGP0503-20, ATGP0503-50, ATGP0503-100, ATGP0503-250, ATGP0503-500, ATGP0503-1

Lindskog S., et al (1997) Pharmacol Ther, 74 (1) :1-20. ; ; Sawaya MR., et al (2006) J Biol Chem. 281 (11) :7546-55;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Enzymes & Proteases

AAH04897

P07451

Human

MAKEWGYASH NGPDHWHELF PNAKGENQSP IELHTKDIRH DPSLQPWSVS YDGGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL VHWNPKYNTF KEALKQRDGI AVIGIFLKIG HENGEFQIFL DALDKIKTKG KEAPFTKFDP SCLFPACRDY WTYQGSFTTP PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL VSNWRPPQPI NNRVVRASFK