Recombinant humaan lactaatdehydrogenase A/LDHA-eiwit

LDHA, also known as L-lactate dehydrogenase A chain, is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. Recombinant LDHA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

38.8 kDa (352aa) confirmed by MALDI-TOF

Lactate Dehydrogenase A, LDHA, L-lactate dehydrogenase A chain, GSD11, LDH1, LDHM, PIG19, L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein, LDH muscle subunit, M LDH, L lactate dehydrogenase A chain, l7R2, Lactate dehydrogenase 1, A chain, Lactate dehydrogenase A, Lactate dehydrogenase A4, Lactate dehydrogenase M, LDH muscle subunit, OTTMuSP00000017774, Proliferation-inducing gene 19, Renal carcinoma antigen NY-REN-59., ATGP0488-10 µg, ATGP0488-20 µg, ATGP0488-50 µg, ATGP0488-100 µg, ATGP0488-250 µg, ATGP0488-500 µg, ATGP0488-1 mg, ATGP0488-10, ATGP0488-20, ATGP0488-50, ATGP0488-100, ATGP0488-250, ATGP0488-500, ATGP0488-1

Gosti F, et al. (1987) Proc. Natl. Acad. Sci. u.S.A. 84 (4) :1000-4; ; Chung FZ, et al. (1985) Biochem. J. 231 (3) :537-41.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Redox Proteins

NP_005557

P00338

Human

MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF