Recombinant humaan LAMTOR4-eiwit

LAMTOR4 is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Recombinant human LAMTOR4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ragulator complex protein LAMTOR4, C7orf59

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

13.1 kDa (122aa) confirmed by MALDI-TOF

LAMTOR4, Ragulator complex protein LAMTOR4, C7orf59, ATGP2791-10 µg, ATGP2791-20 µg, ATGP2791-50 µg, ATGP2791-100 µg, ATGP2791-250 µg, ATGP2791-500 µg, ATGP2791-1 mg, ATGP2791-10, ATGP2791-20, ATGP2791-50, ATGP2791-100, ATGP2791-250, ATGP2791-500, ATGP2791-1

Bar-Peled L., et al. (2011) Cell. 150:1196-1208; ; Burkard T.R., et al. (2012) BMC Syst. Biol. 5:17-17

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_001008396

Q0VGL1

Human

MGSSHHHHHH SSGLVPRGSH MGSMTSALTQ GLERIPDQLG YLVLSEGAVL ASSGDLENDE QAASAISELV STACGFRLHR GMNVPFKRLS VVFGEHTLLV TVSGQRVFVV KRQNRGREPI DV