Recombinant humaan Langerine/CD207-eiwit

CD207 also known as C-type lectin domain family 4 member K. is expressed in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. Additionally it's known by the name C-type lectin domain family 4 member K (CD antigen CD207) . It is also expressed in several other dendritic cell types including dermal CD103+ DCs and splenic CD8+ DCs. Langerin is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Recombinant human CD207, fused to His-tag at N-terminus, was expressed in E. coli.

C-type lectin domain family 4 member K, C-type lectin domain family 4 member K, CLEC4K

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.2 kDa (287aa)

Langerin, CD207, C-type lectin domain family 4 member K, C-type lectin domain family 4 member K, CLEC4K, ATGP3047-10 µg, ATGP3047-20 µg, ATGP3047-50 µg, ATGP3047-100 µg, ATGP3047-250 µg, ATGP3047-500 µg, ATGP3047-1 mg, ATGP3047-10, ATGP3047-20, ATGP3047-50, ATGP3047-100, ATGP3047-250, ATGP3047-500, ATGP3047-1

Feinberg H., et al. (2013) J. Biol. Chem. 288 (52), 36762-36771; ; Lauc G., et al. (2013) PLoS Genet. 9 (1), E1003225;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_056532

Q9UJ71

Human

MGSSHHHHHH SSGLVPRGSH MGSPRFMGTI SDVKTNVQLL KGRVDNISTL DSEIKKNSDG MEAAGVQIQM VNESLGYVRS QFLKLKTSVE KANAQIQILT RSWEEVSTLN AQIPELKSDL EKASALNTKI RALQGSLENM SKLLKRQNDI LQVVSQGWKY FKGNFYYFSL IPKTWYSAEQ FCVSRNSHLT SVTSESEQEF LYKTAGGLIY WIGLTKAGME GDWSWVDDTP FNKVQSARFW IPGEPNNAGN NEHCGNIKAP SLQAWNDAPC DKTFLFICKR PYVPSEP