Recombinant humaan Langerine/CD207-eiwit

CD207, also known as C-type lectin domain family 4 member K, is a type 2 transmembrane glycoprotein. This protein is used as a marker for Langerhans cell which represent the immature dendritic cells in the epidermis. Also, it internalizes endogenous proteins such as type 1 procollagen. Internalization by Langerhans cell is thought to lead to suppression of self reactions. CD207 meditates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae. Recombinant human CD207, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

C-type lectin domain family 4 member K, Langerin, CLEC4K

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

30.9 kDa (273aa)

Langerin, CD207, C-type lectin domain family 4 member K, Langerin, CLEC4K, ATGP3330-10 µg, ATGP3330-20 µg, ATGP3330-50 µg, ATGP3330-100 µg, ATGP3330-250 µg, ATGP3330-500 µg, ATGP3330-1 mg, ATGP3330-10, ATGP3330-20, ATGP3330-50, ATGP3330-100, ATGP3330-250, ATGP3330-500, ATGP3330-1

Ward EM., et al. (2006) J. Biol. Chem. 281:15450-15456.; ; Chabrol E., et al. (2012) PLoS ONE. 7:E50722.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_056532

Q9UJ71

Human

Ala

PRFMGTI SDVKTNVQLL KGRVDNISTL DSEIKKNSDG MEAAGVQIQM VNESLGYVRS QFLKLKTSVE KANAQIQILT RSWEEVSTLN AQIPELKSDL EKASALNTKI RALQGSLENM SKLLKRQNDI LQVVSQGWKY FKGNFYYFSL IPKTWYSAEQ FCVSRNSHLT SVTSESEQEF LYKTAGGLIY WIGLTKAGME GDWSWVDDTP FNKVQSARFW IPGEPNNAGN NEHCGNIKAP SLQAWNDAPC DKTFLFICKR PYVPSEP