Recombinant humaan LAP3-eiwit

LAP3 also known as cytosol aminopeptidase. LAP3 presumably involved in the processing and regular turnover of intracellular proteins. This protein catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. It release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Recombinant human LAP3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytosol aminopeptidase, HEL-S-106, LAP, LAPEP, PEPS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

58.3 kDa (539aa)

LAP3, Cytosol aminopeptidase, HEL-S-106, LAP, LAPEP, PEPS, ATGP3029-10 µg, ATGP3029-20 µg, ATGP3029-50 µg, ATGP3029-100 µg, ATGP3029-250 µg, ATGP3029-500 µg, ATGP3029-1 mg, ATGP3029-10, ATGP3029-20, ATGP3029-50, ATGP3029-100, ATGP3029-250, ATGP3029-500, ATGP3029-1

Hendrickson SL., et al. (2010) PLoS ONE 5 (9), E12862

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 50% glycerol, 5mM DTT, 1mM EDTA

Metabolism

NP_056991.2

P28838

Human

MFLLPLPAAG RVVVRRLAVR RFGSRSLSTA DMTKGLVLGI YSKEKEDDVP QFTSAGENFD KLLAGKLRET LNISGPPLKA GKTRTFYGLH QDFPSVVLVG LGKKAAGIDE QENWHEGKEN IRAAVAAGCR QIQDLELSSV EVDPCGDAQA AAEGAVLGLY EYDDLKQKKK MAVSAKLYGS GDQEAWQKGV LFASGQNLAR QLMETPANEM TPTRFAEIIE KNLKSASSKT EVHIRPKSWI EEQAMGSFLS VAKGSDEPPV FLEIHYKGSP NANEPPLVFV GKGITFDSGG ISIKASANMD LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV RAKNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVILNAA TLTGAMDVAL GSGATGVFTN SSWLWNKLFE ASIETGDRVW RMPLFEHYTR QVVDCQLADV NNIGKYRSAG ACTAAAFLKE FVTHPKWAHL DIAGVMTNKD EVPYLRKGMT GRPTRTLIEF LLRFSQDNA