Recombinant humaan LASP1-eiwit

LASP1, also known as LIM and SH3 domain protein 1, plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types. Recombinant human LASP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

LIM and SH3 domain protein 1, Lasp-1, MLN50

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

32.3 kDa (285aa) confirmed by MALDI-TOF

LASP1, LIM and SH3 domain protein 1, Lasp-1, MLN50, ATGP1386-10 µg, ATGP1386-20 µg, ATGP1386-50 µg, ATGP1386-100 µg, ATGP1386-250 µg, ATGP1386-500 µg, ATGP1386-1 mg, ATGP1386-10, ATGP1386-20, ATGP1386-50, ATGP1386-100, ATGP1386-250, ATGP1386-500, ATGP1386-1

Tomasetto C., et al. (1995) FEBS Lett. 373:245-249; ; Choudhary C., et al. (2002) Science. 325:834-840

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Cytoskeleton

NP_006139

Q14847

Human

MNPNCA RCGKIVYPTE KVNCLDKFWH KACFHCETCK MTLNMKNYKG YEKKPYCNAH YPKQSFTMVA DTPENLRLKQ QSELQSQVRY KEEFEKNKGK GFSVVADTPE LQRIKKTQDQ ISNIKYHEEF EKSRMGPSGG EGMEPERRDS QDGSSYRRPL EQQQPHHIPT SAPVYQQPQQ QPVAQSYGGY KEPAAPVSIQ RSAPGGGGKR YRAVYDYSAA DEDEVSFQDG DTIVNVQQID DGWMYGTVER TGDTGMLPAN YVEAI