Recombinant humaan LC3B/MAP1LC3B-eiwit

Microtubule-associated proteins 1A/1B light chain 3B, also known as MAP1LC3B, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. MAP1LC3B may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. Recombinant human MAP1LC3B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Microtubule associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1ALC3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.2 kDa (140aa) confirmed by MALDI-TOF

MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, ATG8F, LC3B, MAP1A/1BLC3, ATGP0876-10 µg, ATGP0876-20 µg, ATGP0876-50 µg, ATGP0876-100 µg, ATGP0876-250 µg, ATGP0876-500 µg, ATGP0876-1 mg, ATGP0876-10, ATGP0876-20, ATGP0876-50, ATGP0876-100, ATGP0876-250, ATGP0876-500, ATGP0876-1

Behrends C., et al. (2010) Nature. 466 (7302) :68-76.; ; Kabeya Y., et al. (2000) EMBO J. 19 (21) :5720-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl

Cytoskeleton

NP_073729.1

Q9GZQ8

Human

MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG