Recombinant humaan LCH-L5/uch37 eiwit

Ubiquitin carboxyl-terminal hydrolase isozyme L5 (uCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human uCHL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity > 500 pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.

Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, INO80 complex subunit R, INO80R, UCH37, CGI-70, AD-019

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity: > 500pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.

39.7 kDa (349aa) confirmed by MALDI-TOF

UCH-L5, UCHL5, UCH-37, UCH37, UCH L5, UCH 37, Ubiquitin thioesterase L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin carboxyl terminal hydrolase isozyme L5, Ubiquitin C terminal hydrolase UCH37, INO80R, INO80 complex subunit R, CGI-70, CGI70, CGI 70, AD-019, AD019, AD 019, ATGP1069-10 µg, ATGP1069-20 µg, ATGP1069-50 µg, ATGP1069-100 µg, ATGP1069-250 µg, ATGP1069-500 µg, ATGP1069-1 mg, ATGP1069-010, ATGP1069-020, ATGP1069-050, ATGP1069-100, ATGP1069-250, ATGP1069-500, ATGP1069-01M

Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002; ; Nishio K, et al. (2009) Biochem Biophys Res Commun. 390 (3) :855-60.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200 mM NaCl, 0.1mM PMSF, 2mM EDTA

Ubiquitination

NP_057068

Q9Y5K5

Human

MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK