Recombinant humaan LCMT1-eiwit

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A. Recombinant human LCMT7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Leucine carboxyl methyltransferase 1, CGI-68, LCMT, PPMT1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

40.9 kDa (358aa) confirmed by MALDI-TOF

LCMT1, Leucine carboxyl methyltransferase 1, CGI-68, LCMT, PPMT1, ATGP1346-10 µg, ATGP1346-20 µg, ATGP1346-50 µg, ATGP1346-100 µg, ATGP1346-250 µg, ATGP1346-500 µg, ATGP1346-1 mg, ATGP1346-10, ATGP1346-20, ATGP1346-50, ATGP1346-100, ATGP1346-250, ATGP1346-500, ATGP1346-1

Lee JA., et al. (2007) J Biol Chem. 282 (42) :30974-84.; ; De Baere I., et al. (1999) Biochemistry. 38 (50) :16539-47.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Cancer

NP_057393

Q9UIC8

Human

MATRQR ESSITSCCST SSCDADDEGV RGTCEDASLC KRFAVSIGYW HDPYIQHFVR LSKERKAPEI NRGYFARVHG VSQLIKAFLR KTECHCQIVN LGAGMDTTFW RLKDEDLLPS KYFEVDFPMI VTRKLHSIKC KPPLSSPILE LHSEDTLQMD GHILDSKRYA VIGADLRDLS ELEEKLKKCN MNTQLPTLLI AECVLVYMTP EQSANLLKWA ANSFERAMFI NYEQVNMGDR FGQIMIENLR RRQCDLAGVE TCKSLESQKE RLLSNGWETA SAVDMMELYN RLPRAEVSRI ESLEFLDEME LLEQLMRHYC LCWATKGGNE LGLKEITY