Recombinant humaan Legumaisparaginyl Endopeptidase-eiwit

LGMN, also known as legumain, is a cysteine endopeptidase that shows strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. It required for normal lysosomal protein degradation in renal proximal tubules. They are required for normal degradation of internalized EGFR and play a role in the regulation of cell proliferation via its role in EGFR degradation. Recombinant human LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

LGMN, AEP, LGMN1, PRSC1

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

48.4 kDa (422aa)

Legumain, Asparaginyl Endopeptidase, LGMN, AEP, LGMN1, PRSC1, ATGP3185-10 µg, ATGP3185-20 µg, ATGP3185-50 µg, ATGP3185-100 µg, ATGP3185-250 µg, ATGP3185-500 µg, ATGP3185-1 mg, ATGP3185-10, ATGP3185-20, ATGP3185-50, ATGP3185-100, ATGP3185-250, ATGP3185-500, ATGP3185-1

Dall E., et al. (2012) Proc Natl Acad Sci U S A. 110:10940-10945.; ; Chen JM., et al. (1996) J Biol Chem. 272:8090-8098.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_005597

Q99538

Human