Recombinant humaan LIF-eiwit

Leukemia inhibitory factor, also known as LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Recombinant human LIF protein, fused to His GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Leukemia inhibitory factor, CDF, HILDA, DIA, D FACTOR, MLPLI, Emfilermin

E.coli

Human

His-GST-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47.2 kDa (415aa) confirmed by MALDI-TOF

LIF, Leukemia inhibitory factor, CDF, HILDA, DIA, D FACTOR, MLPLI, Emfilermin, ATGP0756-10 µg, ATGP0756-20 µg, ATGP0756-50 µg, ATGP0756-100 µg, ATGP0756-250 µg, ATGP0756-500 µg, ATGP0756-1 mg, ATGP0756-10, ATGP0756-20, ATGP0756-50, ATGP0756-100, ATGP0756-250, ATGP0756-500, ATGP0756-1

Gillett N A., et al. (1993) Growth Factors. 9:301-305.; ; Stahl N., et al. (1994) J Neurobiol. 25:1454-1466.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl

Growth factors

NP_002300

P15018

Human

MHHHHHHMSP ILGYWKIKGL VQPTRLLLEY LEEKYEEHLY ERDEGDKWRN KKFELGLEFP NLPYYIDGDV KLTQSMAIIR YIADKHNMLG GCPKERAEIS MLEGAVLDIR YGVSRIAYSK DFETLKVDFL SKLPEMLKMF EDRLCHKTYL NGDHVTHPDF MLYDALDVVL YMDPMCLDAF PKLVCFKKRI EAIPQIDKYL KSSKYIAWPL QGWQATFGGG DHPPKSDLVP RGSHMSPLPI TPVNATCAIR HPCHNNLMNQ IRSQLAQLNG SANALFILYY TAQGEPFPNN LDKLCGPNVT DFPPFHANGT EKAKLVELYR IVVYLGTSLG NITRDQKILN PSALSLHSKL NATADILRGL LSNVLCRLCS KYHVGHVDVT YGPDTSGKDV FQKKKLGCQL LGKYKQIIAV LAQAF