Recombinant humaan LIF-eiwit

LIF, as known as leukemia inhibitory factor, is a pleiotropic glycoprotein belonging to the LI6 family of cytokines. This protein is involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, embryogenesis and inflammation. It is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. Also, it can function as an autocrine growth factor in some pancreatic cancers, but induced differentiation in the leukemic cell line M1. Recombinant human LIF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human LIF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 0.5 ng/mL.

Leukemia inhibitory factor isoform1, LIF, CDF, DIA, HILDA, MLPLI

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.5 ng/mL.

20.8 kDa (189aa)

LIF, Leukemia inhibitory factor isoform1, LIF, CDF, DIA, HILDA, MLPLI, ATGP3533-10 µg, ATGP3533-20 µg, ATGP3533-50 µg, ATGP3533-100 µg, ATGP3533-250 µg, ATGP3533-500 µg, ATGP3533-1 mg, ATGP3533-10, ATGP3533-20, ATGP3533-50, ATGP3533-100, ATGP3533-250, ATGP3533-500, ATGP3533-1

Kuphal S., et al, (2013) Exp. Mol. Pathol. 95:156-165.; ; Liu SC., et al, (2013) J. Clin. Invest. 123:5269-5283.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_002300

P15018

Human

Ala

SPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLA QAF