Recombinant humaan LIN-28A-eiwit

Lin-28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. It is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. It has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Recombinant Lin28 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Zinc finger CCHC domain-containing protein 1, ZCCHC1, Tex17, RNA binding protein lin 28, LIN28A, LIN-28, Lin 28 homolog A, Lin 28 homolog, FLJ12457, CSDD1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

21.1 kDa (191aa) confirmed by MALDI-TOF

Zinc finger CCHC domain-containing protein 1 AL024421.Lin 28, Zinc finger CCHC domain-containing protein 1, Zinc finger CCHC domain containing protein 1, ZCCHC1, Tex17, RNA binding protein lin 28, LIN28A, LIN-28A, LIN-28, LIN28, LIN 28, LIN 28A, LIN 28 A, Lin 28 homolog A, Lin 28 homolog, FLJ12457, CSDD1, "Lin 28 homolog (C, elegans) ", ATGP0412-10 µg, ATGP0412-20 µg, ATGP0412-50 µg, ATGP0412-100 µg, ATGP0412-250 µg, ATGP0412-500 µg, ATGP0412-1 mg, ATGP0412-010, ATGP0412-020, ATGP0412-050, ATGP0412-100, ATGP0412-250, ATGP0412-500, ATGP0412-01M

Moss E.G., et al. (2003) Dev. Biol. 258:432-442; ; Heo I., et al. (2009) Cell. 138:696-708.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_078950

Q9H9Z2

Human

MGSSHHHHHH SSGLVPRGSH RSMGICKWFN VRMGFGFLSM TARAGVALDP PVDVFVHQSK LHMEGFRSLK EGEAVEFTFK KSAKGLESIR VTGPGGVFCI GSERRPKGKS MQKRRSKGDR CYNCGGLDHH AKECKLPPQP KKCHFCQSIS HMVASCPLKA QQGPSAQGKP TYFREEEEEI HSPTLLPEAQ N