Recombinant humaan LIN-28B eiwit

Lin28B belongs to the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B is acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Recombinant human Lin28B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein lin-28 homolog B, CSDD2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

29.5 kDa (273aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

LIN-28B, LIN 28B, LIN28B, Protein lin-28 homolog B, CSDD2, ATGP1754-10 µg, ATGP1754-20 µg, ATGP1754-50 µg, ATGP1754-100 µg, ATGP1754-250 µg, ATGP1754-500 µg, ATGP1754-1 mg, ATGP1754-010, ATGP1754-020, ATGP1754-050, ATGP1754-100, ATGP1754-250, ATGP1754-500, ATGP1754-01M

Heo I., et al. (2008) Mol. Cell 32:276-284; ; Richards M, et al. (2004) Stem Cells 22 (1) : 51-64.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol,2mM DTT

Cancer

NP_001004317

Q6ZN17

Human

MAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT