Recombinant humaan LIN-7B eiwit

LIN7B, also known as VELI2, belongs to the Velis family. Velis are a family of small synaptic proteins that interact with other proteins at the post-synaptic density (PSD) of neuronal synapses. Velis contain the PDZ motif involved in recruiting cell adhesion molecules, receptors, and channels. Lin7B is ubiquitously expressed with high expression in brain, liver, and testis. It localized at the synaptic junctions in neurons. Lin7B bind to CASK, a neurexin-binding protein highly concentrated in synapses, and Mint1, a binding partner with a vesicle trafficking protein. Recombinant human LIN7B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein lin-7 homolog B, LIN-7B, MALS-2, MALS2, VELI2, Mammalian lin-seven protein 2, Vertebrate lin-7 homolog 2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.3 kDa (230aa) confirmed by MALDI-TOF

Vertebrate lin-7 homolog 2, Vertebrate lin7 homolog 2, Vertebrate lin 7 homolog 2, VELI2, Protein lin-7 homolog B, Protein lin7 homolog B, Protein lin 7 homolog B, Mammalian lin-seven protein2, Mammalian lin-seven protein 2, Mammalian lin seven protein2, Mammalian lin seven protein 2, MALS-2, MALS2, MALS 2, LIN-7B, LIN7B, LIN 7B, ATGP1825-10 µg, ATGP1825-20 µg, ATGP1825-50 µg, ATGP1825-100 µg, ATGP1825-250 µg, ATGP1825-500 µg, ATGP1825-1 mg, ATGP1825-010, ATGP1825-020, ATGP1825-050, ATGP1825-100, ATGP1825-250, ATGP1825-500, ATGP1825-01M

Okamoto M., et al. (1997) J Biol Chem. 272:31459-31464-293; ; Irie M., et al. (1999) Oncogene. 18:2811-2817.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Signal Transduction

NP_071448

Q9HAP6

Human

MGSSHHHHHH SSGLVPRGSH MGSMAALVEP LGLERDVSRA VELLERLQRS GELPPQKLQA LQRVLQSRFC SAIREVYEQL YDTLDITGSA EIRAHATAKA TVAAFTASEG HAHPRVVELP KTDEGLGFNI MGGKEQNSPI YISRVIPGGV ADRHGGLKRG DQLLSVNGVS VEGEQHEKAV ELLKAAQGSV KLVVRYTPRV LEEMEARFEK MRSARRRQQH QSYSSLESRG