Recombinant humaan lipocaline-2/NGAL-eiwit

Neutrophil gelatinase-associated lipocalin (NGAL), also known as LCN2, belongs to the calycin superfamily and Lipocalin family. LCN2 is an iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. The binding of LCN2 to bacterial siderophores is important in the innate immune response to bacterial infection. Also LCN2 functions as a growth factor. LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans. Recombinant human LCN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neutrophil gelatinase-associated lipocalin, Lipocalin-2, MSFI, NGAL, Oncogene 24p3, p25, HNL

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.8 kDa (199aa) confirmed by MALDI-TOF

Lipocalin2, NGAL, Neutrophil gelatinase-associated lipocalin, Lipocalin-2, MSFI, NGAL, Oncogene 24p3, p25, HNL, ATGP0908-10 µg, ATGP0908-20 µg, ATGP0908-50 µg, ATGP0908-100 µg, ATGP0908-250 µg, ATGP0908-500 µg, ATGP0908-1 mg, ATGP0908-10, ATGP0908-20, ATGP0908-50, ATGP0908-100, ATGP0908-250, ATGP0908-500, ATGP0908-1

Flo TH., et al. (2004) Nature 432 (7019) : 917-21; ; Yang J., et al. (2002) Mol. Cell 10:1045-1056

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Immunology

NP_005555

P80188

Human

MGSSHHHHHH SSGLVPRGSH MQDSTSDLIP APPLSKVPLQ QNFQDNQFQG KWYVVGLAGN AILREDKDPQ KMYATIYELK EDKSYNVTSV LFRKKKCDYW IRTFVPGCQP GEFTLGNIKS YPGLTSYLVR VVSTNYNQHA MVFFKKVSQN REYFKITLYG RTKELTSELK ENFIRFSKSL GLPENHIVFP VPIDQCIDG