Recombinant humaan LIRRB1/CD85j/ILT2-eiwit

LILRB1, also known as leukocyte immunoglobulin like receptor B1, is a member of the leukocyte immunoglobulinlike receptor (LIR) family. The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. It is also expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells. It binds to MHC-I as well as non-classical MHC-I molecules and the MHC-I mimetic UL18 encoded by cytomegalovirus. Recombinant human LILRB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human LILRB1 stimulates cell adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cells. This effect is more to 50%.

Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR-1, LIR1, Leukocyte immunoglobulin-like receptor 1, CD85 antigen-like family member J, Immunoglobulin-like transcript 2, ILT-2, ILT2, Ig-like transcript 2, Monocyte/macrophage immunoglobulin-like receptor 7, MIR-7, MIR7, CD_antigen, CD85j, CD85 antigen-like family member J, PIRB, PIR-B, leucocyte Ig-like receptor B1, leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1, monocyte/macrophage immunoglobulin-like receptor 7, myeloid inhibitory receptor 7

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to LILRB1 coated plates 5 µg/mL. This effect is more to 50%.

48.5kDa (446aa)

, ATGP3965-10 µg, ATGP3965-20 µg, ATGP3965-50 µg, ATGP3965-100 µg, ATGP3965-250 µg, ATGP3965-500 µg, ATGP3965-1 mg, ATGP3965-10, ATGP3965-20, ATGP3965-50, ATGP3965-100, ATGP3965-250, ATGP3965-500, ATGP3965-1

Colonna, M. et al. (1997) J. Exp. Med. 186:1809-1818.; ; Cosman, D. et al. (1997) Immunity 7:273-282.; ; Ince, M.N. et al. (2004) Immunology 112:531-542.; ; M Colonna et al. (1998) Immunology 7:3096-3100.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_006660.4

Q8NHL6

Human

Gly24