Recombinant humaan livin bèta-eiwit

Livin isoform beta is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING zinc finger (RZF) domain. Livin isoform beta has direct interaction with several caspases including caspase-3, -7, and -9. This protein inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. Recombinant human Livin beta protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Livin inhibitor of apoptosis isoform beta, BIRC7, KIAP, ML-IAP, MLIAP, RNF50

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.4 kDa (304aa) confirmed by MALDI-TOF

Livin b, Livin inhibitor of apoptosis isoform beta, BIRC7, KIAP, ML-IAP, MLIAP, RNF50, ATGP1416-10 µg, ATGP1416-20 µg, ATGP1416-50 µg, ATGP1416-100 µg, ATGP1416-250 µg, ATGP1416-500 µg, ATGP1416-1 mg, ATGP1416-10, ATGP1416-20, ATGP1416-50, ATGP1416-100, ATGP1416-250, ATGP1416-500, ATGP1416-1

Kasof GM., et al. (2001) . J Biol Chem. 276 (5) :3238-46. ; ; Ashhab Y., et al. (2001) . FEBS Lett. 495 (1-2) :56-60.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 300 mM NaCl, 1mM EDTA

Apoptosis

NP_071444

Q96CA5

Human

MGSSHHHHHH SSGLVPRGSH MGSHMGPKDS AKCLHRGPQP SHWAAGDGPT QERCGPRSLG SPVLGLDTCR AWDHVDGQIL GQLRPLTEEE EEEGAGATLS RGPAFPGMGS EELRLASFYD WPLTAEVPPE LLAAAGFFHT GHQDKVRCFF CYGGLQSWKR GDDPWTEHAK WFPSCQFLLR SKGRDFVHSV QETHSQLLGS WDPWEEPEDA APVAPSVPAS GYPELPTPRR EVQSESAQEP GARDVEAQLR RLQEERTCKV CLDRAVSIVF VPCGHLVCAE CAPGLQLCPI CRAPVRSRVR TFLS