Recombinant humaan LMP7/PSMB8 eiwit

PSMB8, also known as LMP7, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB8 is located in the class II region of the MHC (major histocompatibility complex) . Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. This protein acts as a major component of interferon gamma-induced sensitivity. It plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. Recombinant human PSMB8 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Proteasome (prosome macropain) subunit beta type 8, proteasome (prosome, macropain) subunit, beta type, 8, D6S216, D6S216E, LMP7, MGC1491, PSMB5i

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.4 kDa (229aa)

LMP7, PSMB8, proteasome (prosome macropain) subunit beta type 8, proteasome (prosome, macropain) subunit, beta type, 8, D6S216, D6S216E, LMP7, MGC1491, PSMB5i, ATGP1830-10 µg, ATGP1830-20 µg, ATGP1830-50 µg, ATGP1830-100 µg, ATGP1830-250 µg, ATGP1830-500 µg, ATGP1830-1 mg, ATGP1830-10, ATGP1830-20, ATGP1830-50, ATGP1830-100, ATGP1830-250, ATGP1830-500, ATGP1830-1

Muchamuel T., et al. (2009) Nat. Med. 15:781-787; ; Kitamura A., et al. (2011) J. Clin. Invest. 121:4150-4160;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Ubiquitination

NP_683720.2

P28062

Human

TTTLA FKFQHGVIAA VDSRASAGSY ISALRVNKVI EINPYLLGTM SGCAADCQYW ERLLAKECRL YYLRNGERIS VSAASKLLSN MMCQYRGMGL SMGSMICGWD KKGPGLYYVD EHGTRLSGNM FSTGSGNTYA YGVMDSGYRP NLSPEEAYDL GRRAIAYATH RDSYSGGVVN MYHMKEDGWV KVESTDVSDL LHQYREANQ