Recombinant humaan LPCAT1-eiwit

Lysophosphatidylcholine acyltransferase 1, also known as LPCAT1, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) . Recombinant human LPCAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Lysophosphatidylcholine acyltransferase 1, AYTL2, lpcat, PFAAP3

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

53.4 kDa (479aa)

LPCAT1, Lysophosphatidylcholine acyltransferase 1, AYTL2, lpcat, PFAAP3, ATGP2012-10 µg, ATGP2012-20 µg, ATGP2012-50 µg, ATGP2012-100 µg, ATGP2012-250 µg, ATGP2012-500 µg, ATGP2012-1 mg, ATGP2012-10, ATGP2012-20, ATGP2012-50, ATGP2012-100, ATGP2012-250, ATGP2012-500, ATGP2012-1

Yamazaki T., et al. (2012) Biol Pharm Bull. 35 (9) :1509-15.; ; Kazachkov M., et al. (2001) Lipids. 43 (10) :895-902.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1M urea, 10% glycerol

Metabolism

NP_079106

Q8NF37

Human

SAEKEPE QPPALWRKVV DFLLKAIMRT MWFAGGFHRV AVKGRQALPT EAAILTLAPH SSYFDAIPVT MTMSSIVMKA ESRDIPIWGT LIQYIRPVFV SRSDQDSRRK TVEEIKRRAQ SNGKWPQIMI FPEGTCTNRT CLITFKPGAF IPGAPVQPVV LRYPNKLDTI TWTWQGPGAL EILWLTLCQF HNQVEIEFLP VYSPSEEEKR NPALYASNVR RVMAEALGVS VTDYTFEDCQ LALAEGQLRL PADTCLLEFA RLVRGLGLKP EKLEKDLDRY SERARMKGGE KIGIAEFAAS LEVPVSDLLE DMFSLFDESG SGEVDLRECV VALSVVCRPA RTLDTIQLAF KMYGAQEDGS VGEGDLSCIL KTALGVAELT VTDLFRAIDQ EEKGKITFAD FHRFAEMYPA FAEEYLYPDQ THFESCAETS PAPIPNGFCA DFSPENSDAG RKPVRKKLD