Recombinant humaan LSM1-eiwit

U6 snRNA-associated Sm-like protein LSm1, also known as LSM1 plays a role in replication-dependent histone mRNA degradation and binds specifically to the 3'-terminal u-tract of u6 snRNA. LSM1 protein facilitates RNA protein interactions and structural changes that are required during ribosomal subunit assembly. LSM1 is naturally overexpressed in pancreatic cancer as well as in certain breast cancer cell lines. Recombinant human LSM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

U6 snRNA-associated Sm-like protein LSm1, CASM, Small nuclear ribonuclear CaSm, u6 snRNA-associated Sm-like protein LSm1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.3 kDa (153aa) confirmed by MALDI-TOF

LSM1, u6 snRNA-associated Sm-like protein LSm1, CASM, Small nuclear ribonuclear CaSm, u6 snRNA-associated Sm-like protein LSm1, ATGP0734-10 µg, ATGP0734-20 µg, ATGP0734-50 µg, ATGP0734-100 µg, ATGP0734-250 µg, ATGP0734-500 µg, ATGP0734-1 mg, ATGP0734-10, ATGP0734-20, ATGP0734-50, ATGP0734-100, ATGP0734-250, ATGP0734-500, ATGP0734-1

Noueiry A O., et al. (2003) Mol Cell Biol. 23:4094-4106.; ; Kufel J., et al. (2003) J Biol Chem. 278:2147-2156.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Cancer

NP_055277

O15116

Human

MNYMPGTASL IEDIDKKHLV LLRDGRTLIG FLRSIDQFAN LVLHQTVERI HVGKKYGDIP RGIFVVRGEN VVLLGEIDLE KESDTPLQQV SIEEILEEQR VEQQTKLEAE KLKVQALKDR GLSIPRADTL DEY