Recombinant humaan LSM2-eiwit

LSM2 (u6 snRNA-associated Sm-like protein LSm2) belongs to the snRNP Sm proteins family. Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM2 binds specifically to the 3'-terminal u-tract of u6 snRNA. It is involved in pre-mRNA splicing. Recombinant human LSM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

U6 snRNA-associated Sm-like protein LSm2, Protein G7b, Small nuclear ribonuclear protein D homolog, snRNP core Sm-like protein Sm-x5, C6orf28, YBL026W

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

13.4 kDa (119aa) confirmed by MALDI-TOF

C6orf28, G7b, LSm2, Protein G7b, Small nuclear ribonuclear protein D homolog, snRNP, snRNP core Sm-like protein Sm-x5, u6 snRNA-associated Sm-like protein LSm2, YBL026W, ATGP1476-10 µg, ATGP1476-20 µg, ATGP1476-50 µg, ATGP1476-100 µg, ATGP1476-250 µg, ATGP1476-500 µg, ATGP1476-1 mg, ATGP1476-010, ATGP1476-020, ATGP1476-050, ATGP1476-100, ATGP1476-250, ATGP1476-500, ATGP1476-01M

Lehner, Ben, et al. (2004) Genome Res. (united States) 14 (7) : 1315-23.; ; Achsel T., et al. (1999) EMBO J. 18:5789-5802

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 40% glycerol, 200 mM NaCl

Epigenomics (Transcription & Translation)

NP_067000

Q9Y333

Human

MGSSHHHHHH SSGLVPRGSH MGSHMLFYSF FKSLVGKDVV VELKNDLSIC GTLHSVDQYL NIKLTDISVT DPEKYPHMLS VKNCFIRGSV VRYVQLPADE VDTQLLQDAA RKEALQQKQ