Recombinant humaan LSM4-eiwit

LSM4, also called u6 snRNA-associated Sm-like protein or glycine-rich protein (GRP), forms donut-shaped heptameric complexes that are involved in various steps of RNA metabolism. It facilitates RNA protein interactions and structural changes that are required during ribosomal subunit assembly. It binds specifically to the 3'-terminal u-tract of u6 snRNA. Recombinant human LSM4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

U6 snRNA-associated Sm-like protein LSm4, YER112W

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.5 kDa (159aa) confirmed by MALDI-TOF

LSM4, u6 snRNA-associated Sm-like protein LSm4, YER112W, ATGP0860-10 µg, ATGP0860-20 µg, ATGP0860-50 µg, ATGP0860-100 µg, ATGP0860-250 µg, ATGP0860-500 µg, ATGP0860-1 mg, ATGP0860-10, ATGP0860-20, ATGP0860-50, ATGP0860-100, ATGP0860-250, ATGP0860-500, ATGP0860-1

Hirsch E., et al. (2000), Mol. Cell. Biol., 20: 1055-1062.; ; Brahms H.., et al. (2001) . RNA., 7: 1531-1542;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_036453

Q9Y4Z0

Human

MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ