Recombinant humaan LTA/TNF-beta-eiwit

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant TNF beta protein was expressed in E. coli and purified by using conventional chromatography techniques.

Human TNF-beta induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, TNFB, TNFSF1, TNF superfamily, member 1

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 1 ng/mL.

20.9 kDa (192aa) confirmed by MALDI-TOF

Lymphotoxin a1, TNF b, Lymphotoxin alpha, CLTalpha, Coley's toxin, DIF, Differentiation inducing factor, Hemorrhagic factor, LT, LT alpha, LTA, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Natural killer cytotoxic factor, Necrosin, NKCF, OTTHuMP00000037612, OTTHuMP00000037613, TNF B, TNF superfamily member 1, TNFB, TNFbeta, TNFSF 1, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1, Tumor necrosis factor superfamily member 1, Lymphotoxin alpha, ATGP0517-10 µg, ATGP0517-20 µg, ATGP0517-50 µg, ATGP0517-100 µg, ATGP0517-250 µg, ATGP0517-500 µg, ATGP0517-1 mg, ATGP0517-10, ATGP0517-20, ATGP0517-50, ATGP0517-100, ATGP0517-250, ATGP0517-500, ATGP0517-1

Messer G, et al. (1991) J Exp Med. 173 (1) :209-19; ; Banner DW, et al. (1993) Cell. 73 (3) :431-45.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Hormones & Cytokines

NP_001153212

P01374

Human

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL